Provided are a module for real-time thermal behavior analysis of a secondary cell battery and a method of operating the module. The module includes a region for mounting a sample battery, a region for mounting a reference battery, and a housing covering the two regions and having an adiabatic characteristic. The region for mounting the sample battery is defined by two partitions facing each other. In addition, the region for mounting the reference battery is defined by two partitions facing each other. The region for mounting the sample battery is a region for vertically or horizontally mounting the sample battery. The region for mounting the reference battery is a region for vertically or horizontally mounting the reference battery.