A display apparatus includes a fixing component and a display screen having a first pixel region and a second pixel region. An aligning mark line is provided at a joint of the first and second pixel regions. A method for calibrating a display region of the display apparatus includes: capturing a connecting part of the display screen and the fixing component to obtain a first image; determining whether the aligning mark line is shifted with respect to the fixing component; and generating a correction instruction when the aligning mark line is shifted with respect to the fixing component, wherein the correction instruction includes activating a pixel row of the second pixel region where the aligning mark line is shifted with respect to the fixing component to substitute for a pixel row of the first pixel region shifted to a connecting region of the fixing component.