Embodiments of the present disclosure provide a display substrate, a display device, and a method for manufacturing a display substrate. The display substrate includes: a plurality of bearing parts for supporting display assemblies; and a connecting beam located between two bearing parts and connected to the two bearing parts. The connecting beam includes at least one stretchable portion with a hollow portion provided inside the stretchable portion, so that the stretchable portion is deformable in a first direction and a second direction, and the second direction is perpendicular to the first direction.