A bioptic barcode reader is disclosed for selective use of illumination color to capture appropriate data. The bioptic barcode reader includes a housing and a primary imager positioned within the housing, configured to scan a target object during a first time period. The bioptic barcode reader further includes a primary illumination source positioned within the housing configured to emit primary illumination in a primary wavelength range during the first time period. The bioptic barcode reader further includes a secondary imager configured to capture one or more images of a target object during a second time period. The bioptic barcode reader further includes a secondary illumination source configured to emit secondary illumination in a secondary wavelength range during the second time period, wherein the second time period and first time period are interleaved and the secondary wavelength range is different from the primary wavelength range.