A start signal generation circuit, a driving method and a display device are provided. The start signal generation circuit includes: a pull-down node control sub-circuit; a pull-up control node control sub-circuit, configured to control a potential of the pull-up control node under the control of voltage signals from a first clock signal input terminal, a second clock signal input terminal, and the 2nth clock signal input terminal; a pull-up node control sub-circuit; a storage sub-circuit, connected between the pull-up node PU and a start signal output terminal; and a start signal output sub-circuit, where n is an integer larger than 1, and smaller than or equal to N, N is an integer larger than 1.