Example methods are provided for port mirroring based on remote direct memory access (RDMA) in a software-defined networking (SDN) environment. One example method may comprise obtaining configuration information associated with a port mirroring session between a source logical port supported by a source host and a destination logical port supported by a destination host, and establishing an RDMA-based connection between the source and destination hosts. The method may also comprise: in response to detecting a packet passing through the source logical port, generating a mirrored packet based on the detected packet, and storing, in source memory associated with the source host, the mirrored packet in association with destination information identifying the destination logical port or destination host. The method may further comprise transferring the mirrored packet from the source memory to destination memory associated with the destination host via the RDMA-based connection.