A controlling method of a user interface and an electronic device are provided. A touch element includes a start area, a trigger area and a track area connecting the start area and the trigger area. The controlling method of the user interface includes following steps: entering a startup interface display mode according to the touch behavior performed on the touch element; generating continuous touch data in response to the touch behavior when the touch behavior moves from the start area to the track area and an animation trigger condition is satisfied, activating an animation mode according to the continuous touch data; and generating the continuous touch data in response to the touch behavior when the touch behavior moves from the start area to the track area and from the track area to the trigger area, and opening a user interface according to the continuous touch data.