A pixel arrangement structure, a display substrate, and a display device. The pixel arrangement structure includes pixel groups extending in a first direction and arranged in a second direction, and each pixel group includes a first sub-pixel row, a second sub-pixel row, and a third sub-pixel row. The first sub-pixel row includes a plurality of first sub-pixels, the second sub-pixel row includes a plurality of second sub-pixel pairs, and the third sub-pixel row includes a plurality of third sup-pixels (310). The pitches of the plurality of first sub-pixels, the plurality of second sub-pixel pairs, and the plurality of third sub-pixels in the first direction are same. In each pixel group, a line connecting the centers of a first sub-pixel and a third sub-pixel adjacent to each other are substantially parallel to the second direction.