Invention Grant
- Patent Title: Yeast expressing saccharolytic enzymes for consolidated bioprocessing using starch and cellulose
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Application No.: US16426563Application Date: 2019-05-30
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Publication No.: US11193130B2Publication Date: 2021-12-07
- Inventor: Elena Brevnova , John E. McBride , Erin Wiswall , Kevin S. Wenger , Nicky Caiazza , Heidi Hau , Aaron Argyros , Frank Agbogbo , Charles F. Rice , Trisha Barrett , John S. Bardsley , Abigail Foster , Anne K. Warner , Mark Mellon , Ryan Skinner , Indraneel Shikhare , Riaan Den Haan , Chhayal V. Gandhi , Alan Belcher , Vineet B. Rajgarhia , Allan C. Froehlich , Kristen M. Deleault , Emily Stonehouse , Shital A. Tripathi , Jennifer Gosselin , Yin-Ying Chiu , Haowen Xu
- Applicant: Lallemand Hungary Liquidity Management LLC , Stellenbosch University
- Applicant Address: HU Budapest; ZA Stellenbosch
- Assignee: Lallemand Hungary Liquidity Management LLC,Stellenbosch University
- Current Assignee: Lallemand Hungary Liquidity Management LLC,Stellenbosch University
- Current Assignee Address: HU Budapest; ZA Stellenbosch
- Agency: Nutter MeClennen & Fish LLP
- Main IPC: C12N15/81
- IPC: C12N15/81 ; C12N9/42 ; C12N9/24 ; C12N15/52 ; C12P7/06

Abstract:
The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured together and used to produce ethanol from biomass feedstock.
Public/Granted literature
- US20200095592A1 YEAST EXPRESSING SACCHAROLYTIC ENZYMES FOR CONSOLIDATED BIOPROCESSING USING STARCH AND CELLULOSE Public/Granted day:2020-03-26
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