Check valve assemblies for fluid flow sets and devices are provided. The check valve assembly includes a housing having an inlet body and an outlet body. The inlet body includes a fluid inlet, a first seal bead having a first height, a second seal bead having a second height and a central channel. The outlet body includes a fluid outlet and a stem having a centering post, the centering post received by the central channel of the inlet body. A seal has an inner ring with a first thickness and an outer ring with a second thickness less than the first thickness. The inner ring is engaged with the first seal bead and the outer ring is engaged with the second seal bead in a fully sealed position of the check valve assembly. Fluid flow sets and methods for manufacturing check valve assemblies are also provided.