The present disclosure is related to a display substrate. The display substrate having a display area comprises a first electrode layer on a base substrate; a second electrode layer on a side of the first electrode layer far away from the base substrate; a light emitting layer between the first electrode layer and the second electrode layer; a pixel definition layer defining a plurality of sub-pixel regions with each of the plurality of sub-pixel regions containing the light emitting layer and the first electrode layer; and a signal line coupled to the second electrode layer. The signal line is substantially within the display area and at a different layer from the second electrode layer.