A phosphor wheel includes a substrate and a luminescence layer. The luminescence layer is disposed on the substrate and includes a glue layer, a plurality of scattering particles, and a plurality of first photoluminescence particles. The scattering particles and the first photoluminescence particles are collectively distributed in the glue layer. At least one of the scattering particles is located between the substrate and at least one of the first photoluminescence particles, and at least another of the first photoluminescence particles is located between the substrate and at least another of the scattering particles.