The present disclosure provides a pixel circuit, a pixel circuit driving method, and a display device. The display device can sense and store an original threshold voltage of a driving transistor in the turn-on stage by designing a pixel structure and detecting time sequence. An accrual threshold voltage of the driving transistor is read in an next turn-on stage according to a detected result in order to perform an internal compensation in real-time, and in order to sense and store a mobility of the driving transistor. As a result, unevenness of display can be improved.