Embodiments of the present invention provide a conductive substrate, a manufacturing method thereof and a display device. The conductive substrate includes a base substrate and a first conductive layer and a second conductive layer disposed on the base substrate, wherein the first conductive layer and the second conductive layer contact with each other, the first conductive layer is configured to be electrically connected with separated parts after the second conductive layer is fractured, and the first conductive layer includes a composite material layer or a nanowire conductive network layer.