The present disclosure discloses a sample acquisition method, a target detection model generation method, a target detection method, a computing device, and a computer readable medium. The sample acquisition method includes: adding a perturbation to a pre-marked sample original box in an original image to obtain a sample selection box, wherein an image framed by the sample original box contains a target; and extracting an image framed by the sample selection box as a sample. The technical solutions of the present disclosure can effectively increase the number of the samples that can be acquired in the original image, and adding a background to the samples can effectively improve the recognition accuracy of the trained target detection model.