- 专利标题: Engineered polymerases for improved sequencing
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申请号: US16567476申请日: 2019-09-11
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公开(公告)号: US11242512B2公开(公告)日: 2022-02-08
- 发明人: Pinar Iyidogan
- 申请人: OMNIOME, INC.
- 申请人地址: US CA San Diego
- 专利权人: OMNIOME, INC.
- 当前专利权人: OMNIOME, INC.
- 当前专利权人地址: US CA San Diego
- 代理机构: Kilpatrick Townsend & Stockton LLP
- 主分类号: C12N9/12
- IPC分类号: C12N9/12 ; C12P19/34 ; C12Q1/6874
摘要:
Provided are nucleic acids encoding engineered polymerases comprising at least one modification in a motif A and/or at least one modification in a motif B of the polymerase and engineered polymerases encoded by the nucleic acids. Also provided are engineered DNA polymerases comprising a variant of SEQ ID NO:1, SEQ ID NO:2, or SEQ ID NO:3, the variant being at least 80% identical to SEQ ID NO:1, SEQ ID NO:2 or SEQ ID NO:3 and comprising an amino acid substitution at one or more positions selected from the group consisting of L408, Y409, P410, R484, A/L485, and I486. Methods, vectors, kits, and compositions comprising the nucleic acids and compositions, methods and kits comprising the engineered polymerases are also provided.
公开/授权文献
- US20200087637A1 ENGINEERED POLYMERASES FOR IMPROVED SEQUENCING 公开/授权日:2020-03-19
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