A light tube with wire guide includes a hollowed tube body, two caps, a wire guide, and a plurality of wires. The two caps respectively cover two ends of the hollowed tube body. The wire guide is located at an outer side wall of one of the two caps. The wire guide includes a guiding slot communicating with the hollowed tube body. The wires are disposed in the wire guide and extended along the guiding slot. One ends of the wires are located in the hollowed tube body or located in one of the two caps, and the other ends of the wires extend to the guiding slot and are exposed out of the hollowed tube body.