Disclosed are a display panel, a preparation method thereof and a display device. The display panel includes: a display substrate having a through hole which runs through the display substrate in a thickness direction; and an anti-reflective film, where the anti-reflective film is disposed on a light-emitting surface of the display substrate, the anti-reflective film includes a light-transmitting structure and an anti-reflective structure, and an orthographic projection of the light-transmitting structure on the display substrate covers the through hole.