The present disclosure discloses a spatial positioning method, a spatial positioning device, a spatial positioning system and a computer readable medium. The spatial positioning method includes: capturing a feature image containing multiple feature markers of a to-be-positioned object; determining coordinate positions of respective feature markers in the feature image in an image coordinate system; traversing the respective feature markers in the image coordinate system according to preset multiple standard vector relations, and arbitrarily selecting at least three of the feature markers to calculate vector relations corresponding to the standard vector relations; determining position information of at least three feature markers according to position information corresponding to the standard vector relations in the image coordinate system when it is determined that the calculated vector relations satisfy any standard vector relation.