A display substrate is provided. The display substrate includes a base substrate; a pixel definition structure on the base substrate; a plurality of light emitting elements respectively in a plurality of first apertures; and a reflective layer at least partially in a respective one of the plurality of first apertures and configured to reflect light laterally emitted from a respective one of the plurality of light emitting elements to exit from a light emitting surface of the respective one of the plurality of light emitting elements. The pixel definition structure includes a first pixel definition layer on the base substrate, and a second pixel definition layer on a side of the first pixel definition layer away from the base substrate. Lateral sides of the first pixel definition layer and the second pixel definition layer have different slope angles with respect to a main surface of the base substrate.