In an embodiment, there is provided a display substrate assembly. The display substrate assembly includes: a base substrate; a light-shielding layer on the base substrate, the light-shielding layer having a plurality of light-shielding elements; and a plurality of polysilicon layers respectively on sides of the plurality of light-shielding elements away from the base substrate; wherein the plurality of light-shielding elements have different sizes such that energy lights reflected and/or refracted through the plurality of light-shielding elements of different sizes respectively generate different thermal energy distributions on the plurality of polysilicon layers corresponding to the plurality of light-shielding elements, causing the plurality of polysilicon layers to have different crystal forms. Meanwhile, a method of manufacturing the display substrate assembly and a display apparatus including the aforementioned display substrate assembly are also provided.