A fastener structure and an assembling method thereof are introduced. The fastener structure includes a head portion and a body portion. The body portion is movably connected to the head portion and includes a connecting section for assembling to an intermediate member. The intermediate member includes a material retaining section and can be further connected to an object. Partial material of the object would move or flow into the material retaining section when the intermediate member is forcefully pressed against the object, so that the intermediate member and the object are connected together. Since the body portion can be assembled to the intermediate member and the intermediate member can be further connected to an object, and the fastener structure can be fastened to and released from a target object, at least two objects can be repeatedly and quickly connected to and disconnect from one another using the fastener structure.