A display substrate, a high-precision metal mask, a display device and a display driving method are provided in the present disclosure. A plurality of pixel repetition units is included in a display region of the display substrate, each pixel repetition unit includes two first sub-pixels, one second sub-pixel and one third sub-pixel, one of the two first sub-pixels is located in a first row, and the other of the two first sub-pixels is located in a third row, the second sub-pixel and the third sub-pixel are arranged in a same second row. A distribution density of the pixel repetition units in the first region is smaller than a distribution density of the pixel repetition units in the second region.