An extraction assembly for extracting content from a bag, said bag having an end mouth formed by two opposing flaps, comprising a separation wall which separates a first environment and a second environment at different contamination classes and which is provided with an opening communicating the first environment and the second environment, closing partitions at the opening movable between a closing position and an opening position, and a gripper to keep closed said flaps and wherein each closing partition has coupling elements to couple with a gripper, wherein said gripper and said coupling elements include a retention device, the spacing apart of partitions causing opening of the jaws and the opening of the end mouth and the communicating of the inside of the bag and of the content with said second environment through said opening.