A system and method for detection of media items matching is disclosed. In an implementation, the method includes identifying a first media item associated with a channel of a first user; determining whether the first media item qualifies as a candidate reference media item based on properties of the first media item and the channel; analyzing content of the first media item to determine whether a first threshold amount of frames of the first media item does not match with any frame of any previously uploaded media item; selecting the first media item as a reference media item; detecting a subsequently uploaded media item that includes at least a second threshold portion of the reference media item; and providing a graphical user interface (GUI) for presentation to the first user, the GUI including the subsequently uploaded media item and one or more actions to be initiated by the first user.