A display substrate includes an insulating substrate, a first gate line, a first lower electrode, a second lower electrode, a first upper electrode, and a second upper electrode. The insulating substrate includes a first pixel region and a second pixel region located at a first direction from the first pixel region. The first gate line extends in a second direction crossing the first direction on the insulating substrate. The first and the second lower electrodes are in the first and the second pixel regions, respectively. The first upper electrode overlaps the first lower electrode in the first pixel region and includes a first slit pattern extending in a third direction different from the first and the second directions. The second upper electrode overlaps the second lower electrode in the second pixel region and includes a second slit pattern extending in a fourth direction different from the first to the third directions.