Pixel driving method for driving pixel unit, display driving method and display substrate are provided. The pixel unit includes pixel driving circuit, including driving transistor, storage capacitor, and data writing circuit, the driving transistor has control electrode coupled to first terminal of the data writing circuit and the storage capacitor, and first electrode coupled to second terminal of the storage capacitor, and second terminal of the data writing circuit is coupled to data line. The pixel driving method includes: loading a data voltage into the data line, and controlling the first and second terminals of the data writing circuit to be connected; controlling the data line to be floating, and maintaining connection between the first and second terminals of the data writing circuit to reduce gate-source voltage of the driving transistor; and controlling the first and second terminals of the data writing circuit to be disconnected.