The present disclosure provides a display substrate and a display device. A display substrate provided by an embodiment of the present disclosure includes: a display region and a peripheral region surrounding the display region; the display region includes: a plurality of gate lines, a plurality of data lines, and a plurality of pixel units, and each of the plurality of pixel units includes a driving transistor and a pixel electrode that are connected to each other; the peripheral region includes: signal lines and at least one electrostatic discharge structure for performing electrostatic discharge on the signal lines.