A touch display substrate, including touch electrodes, touch lines and sub-pixels, each sub-pixel includes a storage capacitor, a driving transistor, a switching element and a light emitting element, a first electrode and a second electrode of the storage capacitor are coupled to a gate electrode and a first electrode of the driving transistor, respectively, and a second electrode of the driving transistor, the switching element and the light emitting element are sequentially coupled in series, each touch electrode is coupled to one corresponding touch line through a via hole in an insulating layer between said each touch electrode and the corresponding touch line, and each touch electrode is formed by the second electrode of the storage capacitor in at least one sub-pixel, during a touch stage, the switching element decouples electrical coupling between the second electrode of the driving transistor and the light emitting element.