Systems and methods for ossification center detection (OCD) and bone age assessment (BAA) may be provided. The method may include obtaining a bone age image of a subject. The method may include generating a normalized bone age image by preprocessing the bone age image. The method may include determining, based on the normalized bone age image, positions of a plurality of ossification centers using an ossification center localization (OCL) model. The method may include estimating, based on the normalized bone age image and information related to the positions of the plurality of ossification centers, a bone age of the subject using a bone age assessment (BAA) model.