The magnetic steel loading and separating device is used for separating magnetic steel strips and large plastic spacers of a magnetic steel plate, and includes a material case, a material pushing mechanism and a material separating mechanism. The material case includes a case body for accommodating the magnetic steel plate, and a discharge port is formed in a side wall of the case body. The material pushing mechanism is used for successively pushing the magnetic steel strips and the large plastic spacers of the magnetic steel plate out of the material case via the discharge port, wherein a discharge position and a take-up position that are provided for the magnetic steel strip are formed outside the material case. The material separating mechanism includes a magnetic steel pushing block that reciprocates between the discharge position and the take-up position of the magnetic steel strip.