Various embodiments are provided for calibration of motion player tracking devices in electronic gaming machines (EGMs). A plurality of object sensors positioned adjacently to a display device of the EGM each record a plurality of data frames inclusive of a position of a plurality of fiducial markers relative to the plurality of object sensors. At predetermined intervals, the plurality of object sensors are calibrated in three-dimensional space using the position of the plurality of fiducial markers relative to the plurality of object sensors within the recorded plurality of data frames.