The present disclosure relates to a communication method and system for converging a 5th-Generation (5G) communication system for supporting higher data rates beyond a 4th-Generation (4G) system with a technology for Internet of Things (IoT). The present disclosure may be applied to intelligent services based on the 5G communication technology and the IoT-related technology, such as smart home, smart building, smart city, smart car, connected car, health care, digital education, smart retail, security and safety services. A method by a first terminal in a wireless communication system is provided. The method includes determining whether to transmit a preamble for an automatic gain control (AGC), determining a slot and at least one symbol in the slot to transmit the preamble for the AGC, in a case in which it is determined to transmit the preamble for the AGC, and transmitting, to a second terminal, the preamble for the AGC in the determined slot and the at least one symbol in the slot.