Disclosed are a transportation device and a fixing seat thereof. The former includes at least one transportation trolley including a base and at least one fixing seat. The base includes a carrying surface having a plurality of first positioning structures. A screen box body is fixed between the fixing seat and the base. The fixing seat includes a plurality of second positioning structures at one side of the fixing seat facing the base. The base includes at least one caster on a side of the base facing away from the carrying surface. Each of the first positioning structures is at least one of a first positioning piece and a first positioning hole, and each of the second positioning structures is at least one of a second positioning hole and a second positioning piece. Each of the first positioning piece defines a first fixing hole configured to receive a fixing plug.