The present application discloses an apparatus for separating and feeding O-rings, which includes a rotatable body including a storage portion and a separating portion; and a helix groove formed on the rotatable body across the storage portion and the separating portion, wherein a width and a depth of the helix groove are adapted to a wire diameter of a O-ring, and pitch of the helix groove on the separating portion increases along a feeding direction from the storage portion to the separating portion, wherein in response to a rotation of the rotatable body, the helix groove conveys a plurality of O-rings hanged on the storage portion to the separating portion to thereby separate the plurality of O-rings away from each other.