The present invention provides a manufacturing method of an array substrate, including steps of: providing a flexible substrate layer, forming a buffer layer, forming an active layer, forming a gate insulating layer, forming a gate layer, forming an interlayer insulating layer, forming a source and drain layer, forming an organic planarization layer, forming an anode layer. An array substrate manufactured by the above manufacturing method, and the array substrate includes laminated a flexible substrate layer, a buffer layer, an active layer, a gate insulating layer, a gate layer, an interlayer insulating layer, a source and drain layer, an organic planarization layer, and an anode layer, which are disposed in a stack.