An optical fiber polishing fixture assembly comprises a fixture base and a clamping assembly. The clamping assembly has first and second base portions, a lever, and a biasing member. Each of the first and second base portions has an inner facing side with a slot and an inner facing surface. The slots are adapted to receive bars on opposing sides of a fiber optic connector and cable assembly. The lever has a first end positioned between the inner facing surfaces and a second end extending outward therefrom. The clamping assembly has a locked position, when the first and second base portions are biased toward one another by the biasing member, and an unlocked position, when the lever overcomes a biasing force of the biasing member and separates the first and second base portions.