Techniques for enabling deduplication-aware load balancing in a distributed storage system are provided. In one set of embodiments, a node of the distributed storage system can receive an I/O (Input/Output) request pertaining to a data block of a storage object stored on a local storage component of the node. The node can further determine whether the I/O request requires insertion of a new entry into a deduplication hash table associated with the local storage component or deletion of an existing entry from the deduplication hash table. If the I/O request requires insertion of a new hash table entry, the node can add an identifier of the data block into a probabilistic data structure associated with the local storage component, where the probabilistic data structure is configured to maintain information regarding distinct data blocks that are likely present in the local storage component. Alternatively, if the I/O request requires deletion of an existing hash table entry, the node can remove the identifier of the data block from the probabilistic data structure.