A random access method, a base station and a User Equipment (UE) are provided. A random access method applied to a base station side includes: receiving, by using a pre-configured first SubCarrier Spacing (SCS), a first random access preamble message transmitted by a UE when the base station instructs the UE to perform an initial access according to a contention-based random access mechanism; transmitting, by using a predetermined second SCS or a third SCS configured by a network, a first Random Access Response (RAR) message to the UE; receiving, by using a predetermined fourth SCS or a fifth SCS configured by the network, a first scheduling transmission message transmitted by the UE; and transmitting, by using a predetermined sixth SCS or a seventh SCS configured by the network, a first collision resolution message to the UE, to complete the initial access.