An electronic device housing may include a display cover layer that overlaps an array of pixels. The cover layer or other portion of the housing may have an opening that forms an optical and audio port. Optical and audio components may be coupled to the port. A port cover may cover some or all of the port. The port cover may include one or more layers of mesh or other materials that allow light and sound to pass while blocking environmental contaminants. The optical components may include an ambient light sensor or other devices that receive light and/or may include devices that emit light. The audio components may include a microphone and/or a speaker. The optical component may emit or receive light that passes through the audio component. A structure in the interior of the housing may form a passageway coupled to the port through which sound and light passes.