A display device including a substrate, a gate driving circuit, at least one ambient light sensing element and at least one ambient light sensing signal line is provided by the present disclosure. The substrate includes a peripheral region, and the gate driving circuit, the ambient light sensing element and the ambient light sensing signal line are disposed in the peripheral region. The ambient light sensing signal line is electrically connected to the ambient light sensing element, wherein the ambient light sensing signal line at least partially overlaps the gate driving circuit in a top view direction of the substrate.