A touch substrate, a manufacturing method thereof, and a display device. The touch substrate includes: a basal substrate; a first touch electrode, a black matrix, and a second touch electrode located on a first surface of the basal substrate; an orthographic projection of the first touch electrode on the basal substrate and an orthographic projection of the second touch electrode on the basal substrate at least partially overlap with each other; the first touch electrode and the second touch electrode are insulated from each other; in an overlapping area of the first touch electrode and the second touch electrode, the black matrix is located between the first touch electrode and the second touch electrode.