A timing controller, a display panel and a driving method are proposed. The display panel includes a display sub-pixel and a virtual sub-pixel. The display sub-pixel includes first sub-pixels, second sub-pixels and third sub-pixels. If the grayscale information of the two adjacent first sub-pixels are the same, then a driving signal of the virtual sub-pixel is the same as a driving signal of a third sub-pixel close to the virtual sub-pixel; otherwise, the driving signal of the virtual sub-pixel is the same as a driving signal of the third sub-pixels, which are outnumbered in the histogram.