A threaded fastening assembly includes a nut body and a screw body. The internal thread of the nut body includes a nut thread crest and a nut thread root. The external thread of the screw body includes a screw thread crest, a screw thread root, a fourth side wall located on one side of the screw thread crest, and a fifth side wall located on the other side of the screw thread crest. A locking notch located on one side of the nut thread crest is arranged on the internal thread. The locking notch includes a first side wall and a bottom wall. One end of the first side wall is connected to the nut thread crest, and the other end of the first side wall is connected to one end of the bottom wall. The bottom wall is spirally distributed around the axis of the nut body.