An object holder with quick-release anchoring capability includes an object carrier having an object carrier base. A vacuum release actuator on the object carrier base is displaceable to separate from the object. An anchor member carried by vacuum release actuator includes outer and inner seal members and an interconnecting vent port. The outer and inner seal members are arranged to form substantially airtight seals with the reference surface and the object, respectively, that define controlled pressure zones. The vent port extends between the outer and inner controlled pressure zones. The inner seal member has a substantially non-folding configuration that prevents excessive seal elongation prior to separation from the object. During operation, the controlled pressure zones can be vented by manipulating the object carrier away from the reference surface to separate the object and the vacuum release actuator until the inner seal member breaks its substantially airtight seal with the object.