The present disclosure relates to a display panel, a method of manufacturing a display panel, and a mask plate. A display panel includes a plurality of first light emitting layers configured to emit a light of a first color when excited; a plurality of second light emitting layers configured to emit a light of a second color when excited; and a plurality of third light emitting layers configured to emit a light of a third color when excited, wherein the first and second light emitting layers are alternately arranged in a first direction and a second direction crossing the first direction, and the third light emitting layers are arranged between the first and second light emitting layers, and each of the first and second light emitting layers includes chamfered corners, so that the first and second light emitting layers do not overlap.