An integrated photo-sensing detection display substrate. The integrated photo-sensing detection display substrate includes a base substrate; a plurality of light emitting elements on the base substrate and configured to emit light, a portion of the light being totally reflected by a surface thereby forming totally reflected light; an addressable diffraction grating layer on a side of the base substrate away from the plurality of light emitting elements, and including a plurality of individually addressable diffraction regions, light diffraction respectively in the plurality of individually addressable diffraction regions being independently controllable; and a photosensor on a side of the addressable diffraction grating layer away from the base substrate and configured to detect light transmitted from one or more of the plurality of individually addressable diffraction regions, thereby detecting fingerprint information.