The present application provides a display substrate, a display panel and a spliced screen, and belongs to the field of display technology. The display substrate in the present application is divided into display and boding regions, and includes a base substrate, and a pixel driving circuit on the base substrate and corresponding to the display region. The display substrate further includes an interlayer insulating layer and a signal lead-in wire on the base substrate. The signal lead-in wire at least includes a first signal lead-in sub-wire on a side of the interlayer insulating layer away from the base substrate. The first signal lead-in sub-wire is connected to a first connection pad in the bonding region for introducing an external signal to the pixel driving circuit. The interlayer insulating layer includes sub-insulating layers defining a multi-stepped structure at a position where the display region is adjacent to the bonding region.