Provided are a color film substrate and manufacturing method thereof, and a display panel and manufacturing method thereof. The color film substrate is divided into a display region, and a non-display region around a periphery of the display region and including an edge region at an edge of the color film substrate. The color film substrate includes base, color resist layer on the base and black matrix layer on the base at least in the display region. In the edge region, the color resist layer includes first sub color resist layer on the base and second sub color resist layer on the first sub color resist layer, the first sub color resist layer allows light in first wavelength range to transmit therethrough, the second sub color resist layer allows light in a second wavelength range to transmit therethrough, and the first wavelength range does not overlap the second wavelength range.