An image positioning system based on upsampling and a method thereof are provided. The image positioning method based on upsampling is to fetch a region image covering a target from a wide region image, determine a rough position of the target, execute an upsampling process on the region image based on neural network data model for obtaining a super-resolution region image, map the rough position onto the super-resolution region image, and analyze the super-resolution region image for determining a precise position of the target. The present disclosed example can significantly improve the efficiency of positioning and effectively reduce the required cost of hardware.